Bioactive Small Molecules
STEMCELL Technologies Dibutyryl-cAMP (Sodium Salt), Size: 100 mg
Dibutyryl-cAMP is a cell-permeable cyclic AMP (cAMP) analog that activates cAMP-dependent protein kinases (Schwede et al.). This product is supplied as the sodium salt of the molecule.DIFFERENTIATION· Suppresses experimental autoimmune encephalomyelitis development by reducing demyelination and mobilizing neural stem cells in the subventricular zone towards the demyelinated plaques (Khezri et al.).· Induces intrinsic axon growth in peripheral and central nervous systems, and morphological differentiation of astrocytes (Knott et al.; Imamura & Ozawa).· Stimulates neurite outgrowth in PC12 cells (Maruoka et al.).
STEMCELL Technologies Ruxolitinib, Size: 1 mg
Ruxolitinib is an ATP mimic that inhibits all Janus-associated kinase (JAK) family kinases with a preference for JAK-1 and JAK-2 over JAK-3 and TYK2 with IC₅₀ values of 3.3, 2.8, 428 and 19 nM respectively (Verstovsek; Quintás-Cardama et al.).CANCER RESEARCH· Suppresses erythroid progenitor colony formation from peripheral blood mononuclear cells of polycythemia vera patients with the constitutively active JAK2 V617F mutation. Reduces malignant cell proliferation and decreases interleukin-6 and TNF-α signaling in a JAK2 V617F-driven mouse model of myeloproliferative disorder (Quintás-Cardama et al.). OTHER· Promotes hair regrowth in a mouse model of alopecia areata (Xing et al.).
STEMCELL Technologies R848, Size: 50 mg
Modify immune responses by mimicking the pathogen-associated molecular patterns that activate immune cells through TLR7 and TLR8 with R848, an agonist of Toll-like receptors (TLRs) 7 and 8. This immune modulator targets osteoclast precursors and inhibits their differentiation into osteoclasts via TLR7 (Miyamoto et al. Cytotechnology, 2012); and induces myeloid differentiation of CD34+ hematopoietic progenitor cells (Sioud et al. J Mol Biol, 2006). R848 is also used in immunology research to trigger the activation of human B cells (Bishop et al. J Immunol, 2000); induce expression of IL-12 and IFN-ɣ in mouse and human peripheral blood cell cultures (Wagner et al. Cell Immunol, 1999); and suppress HIV-1 replication in monocytes (Nian et al. BMC Infect Dis, 2012). R848 is supplied as a crystalline solid with ≥98% purity; Molecular weight 314.4 g/mol; CAS Number 144875-48-9. Visit STEMCELL.com for more information and related products.
STEMCELL Technologies IWR-1-endo, Size: 5 mg
IWR-1-endo potently inhibits WNT signaling by blocking a cell-based WNT/β-catenin pathway reporter response with an IC₅₀ value of 180 nM (Chen et al.). It inhibits WNT-induced accumulation of β-catenin, through stabilization of the destruction complex member AXIN2 (Chen et al.).MAINTENANCE AND SELF-RENEWAL · Promotes self-renewal and maintains pluripotency of human embryonic stem cells and mouse Epi-stem cells when used in combination with CHIR99021 (Kim et al.). DIFFERENTIATION · Promotes differentiation of cardiomyocytes from human pluripotent stem cells (PSCs) that have been induced to mesoderm by addition of Activin A and/or BMP4 (Ren et al.; Willems et al.)· Induces the differentiation of human PSC-derived alveolar epithelial type II (AETII) to AETI cells (Ghaedi et al.).
STEMCELL Technologies BIRB-796, Size: 1 mg
BIRB-796 is a highly potent inhibitor of p38 MAPK (Kd = 0.1 nM) that blocks TNFα release in LPS-stimulated THP-1 cells (IC₅₀ = 18 nM) (Pargellis et al.). At 10 μM, BIRB-796 can also inhibit JNK2α2 in vitro, but at lower concentrations BIRB-796 inhibits p38 MAPK without affecting phosphorylation of JNK substrates (Bain et al.; Kuma et al.).MAINTENANCE AND SELF-RENEWAL· Rescues the self-renewal ability of muscle satellite cells from aged mice (Bernet et al.).· Increases the regenerative capacity of functional aged skeletal muscle stem cells grown in hydrogel (Cosgrove et al.).· Blocks GADD45G-induced differentiation of long-term repopulating hematopoietic stem cells (Thalheimer et al.).· Enhances stem cell activity of cultured umbilical cord blood-derived hematopoietic cells cultured in serum-free medium supplemented with SCF, TPO, and FLT3L (Baudet et al.).· Blocks the osteogenic differentiation effect of myocilin on human mesenchymal stem cells (Kwon et al.).
STEMCELL Technologies CH223191, Size: 10 mg
CH223191 is a potent and specific antagonist of the aryl hydrocarbon receptor (AhR) that blocks activation by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD; IC₅₀ = 0.03 µM; Kim et al.). It prevents the induction of cytochrome P450 1A1 by TCDD in HepG2 cells and in livers of mice (Kim et al.).MAINTENANCE AND SELF-RENEWAL· Induces expansion of human CD34+ hematopoietic stem and progenitor cells in culture (Boitano et al.).
STEMCELL Technologies FK506, Size: 10 mg
FK506 is a macrolide antibiotic with potent immunosuppressive properties (Thomson et al.). It inhibits the calcineurin/NFAT pathway (Martinez-Martinez & Redondo) and binds with high affinity to FK506 binding protein 12 (FKBP-12) (Thomson et al.).MAINTENANCE AND SELF-RENEWAL· Promotes the self-renewal of mouse embryonic stem cells independent of leukemia inhibitory factor (LIF), and attenuates differentiation following LIF withdrawal (Li et al.). · In combination with 1-Azakenpaullone (Catalog #74252) and ID-8 (Catalog #72502), enhances the expansion rates of several human pluripotent stem cell lines (Yasuda et al.).IMMUNOLOGY· Inhibits pro-inflammatory cytokine production by activated T cells (Sakuma et al.).
STEMCELL Technologies SAHA, Size: 100 mg
SAHA (suberoylanilide hydroxamic acid) is a potent inhibitor of class I and class II histone deacetylases (HDACs). This compound inhibits the activities of HDAC1 and HDAC3 (IC50 = 10 nM and 20 nM, respectively; Richon et al.). SAHA exhibits antiproliferative properties and can cause cell cycle arrest at G1 (Marks & Breslow). REPROGRAMMING· Improves reprogramming efficiency from human and mouse somatic to induced pluripotent stem cells (Huangfu et al.).CANCER RESEARCH· Downregulates homologous recombination and leads to apoptosis in ovarian cancer cells when used in combination with olaparib (Konstantinopoulos et al.).· SAHA downregulates Nanog expression, which leads to proliferation inhibition in head and neck cancer stem cells (Kumar et al.).
STEMCELL Technologies Paclitaxel, Size: 25 mg
Inhibit microtubule formation with Paclitaxel. This diterpene alkaloid inhibits tumor cell growth in a variety of cancer cell lines such as cervical (HeLa), lung (A549), and breast (MCF-7) carcinomas (Liebmann et al. Br J Cancer, 1993). Paclitaxel is used to inhibit the initiation and outgrowth of neurites in vitro, through microtubule polymerization (Letourneau & Ressler. J Cell Biol, 1984); induce cell cycle arrest at prophase and G1 in various human cancer cell lines (Woods et al. Mol Med, 1995); and initiate apoptosis of cancer cells through various mechanisms such as p53-dependent and -independent pathways, B-cell CLL/lymphoma 2 (BCL-2) family members, cyclin-dependent kinases, and p38 MAPK (Wang et al. Cancer, 2000; Reshkin et al. Clin Cancer Res, 2003). Paclitaxel is supplied as a crystalline solid with ≥98% purity; Molecular weight 853.9 g/mol; CAS Number 33069-62-4. Visit STEMCELL.com for more information and related products.
STEMCELL Technologies All-Trans Retinoic Acid, Size: 500 mg
Activate the retinoic acid receptor (RAR) pathway and subsequently alter gene expression with All-Trans Retinoic Acid (Apfel et al. Proc Natl Acad Sci USA, 1992; Chambon. FASEB J, 1996). This Vitamin A derivative promotes the differentiation of mouse and human pluripotent stem cells to motor neurons (Dimos et al. Science, 2008, Wichterle et al. Cell, 2002); promotes the differentiation of neural stem cells to neurons (Takahashi et al. J Neurobiol, 1999); and promotes terminal differentiation of granulocytes (Collins. Leukemia, 2006). All-Trans Retinoic Acid is also used to differentiate mouse embryonic stem (ES) cells into adipocytes (Dani et al. J Cell Sci, 1997) and ventricular cardiomyocytes (Wobus et al. J Mol Cell Cardiol, 1997). All-Trans Retinoic Acid is supplied as a crystalline solid with ≥98% purity; Molecular weight 300.4 g/mol; CAS Number 302-79-4. Visit STEMCELL.com for more information and related products.
STEMCELL Technologies DMH1, Size: 10 mg
Selectively inhibit activin receptor-like kinase 2 (ALK2) with DMH1 (Hao et al. ACS Chem Biol, 2010). DMH1 suppresses non-small cell lung cancer cell growth, migration, and invasion in vitro (Hao et al. PLoS One, 2014) and inhibits chemotherapeutic drug-induced autophagy response (Sheng et al. Acta Pharm Sin B, 2015). This small molecule also dorsalizes the embryonic axis without disrupting the angiogenic process in early zebrafish embryos (Hao et al. ACS Chem Biol, 2010); induces differentiation of mouse embryonic stem cells to cardiomyocyte progenitor cells (Ao et al. PLoS One, 2012); and induces differentiation of human induced pluripotent stem cells to SOX1 and PAX6 expressing neural precursor cells (Neely et al. ACS Chem Neurosci, 2012). DMH1 is supplied as a crystalline solid with ≥95% purity; Molecular weight 432.5 g/mol; CAS Number 208255-80-5. Visit STEMCELL.com for more information and related products.
STEMCELL Technologies 8-Bromo-cAMP, Size: 10 mg
8-Bromo-cAMP is a membrane-permeable cAMP derivative. It can activate cAMP-dependent protein kinase, with long-acting effects due to its resistance to cAMP phosphodiesterase (Schwede et al.). It can be used to study calcium-mediated pathways (IC₅₀ = 0.84 mM; Xaus et al.).REPROGRAMMING · Improves the reprogramming efficiency of human neonatal foreskin fibroblast (HFF1) cells, in combination with valproic acid (Wang & Adjaye). IMMUNOLOGY · Inhibits M-CSF-dependent proliferation of macrophages (Xaus et al.). · Protects neutrophils against TNF-α-induced apoptosis (Krakstad). CANCER RESEARCH · Induces a proliferative response in an IL-3-dependent leukemic cell line (Barge et al.).· Induces membrane depolarization in pancreatic cancer cell lines (Sorio et al.).
Medchemexpress LLC RSPO1/R-spondin-1 H 50ug
RSPO1 or R-spondin-1 activates the canonical Wnt pathway through ligand binding to the LGR4-6 receptor forming a complex with phosphorylated LRP6 and Frizzled receptors This interaction upregulates target gene expression RSPO1/R-spondin-1 Protein Human (125a a HEK293 His) is the recombinant human-derived RSPO1/R-spondin-1 protein expressed by HEK293 with C-8 His labeled tag
Medchemexpress LLC Complement C3/C3a M 2ug | 2ug
Complement C3/C3a protein activates the complement system playing a central role in both classical and alternative pathways C3b binds covalently to cell surface carbohydrates or immune aggregates while C3a acts as an inflammatory mediator inducing neutrophil chemoattraction and promoting smooth muscle contraction increased vascular permeability and histamine release The shorter isoform of C3a stimulates B-cells Complement C3/C3a Protein Mouse is the recombinant mouse-derived Complement C3/C3a protein expressed by E coli with tag free